| Plant Tissue Culture/Plant Biotechnology
Methods-
Hort/Biotech/Biol 459 (Spring Semesters)
I teach the first half of the course, the second half covers
plant molecular biology techniques and theory, currently taught
by Dr. Ola Sodeinde oas1@psu.edu.
Summary:
Tissue culture is the aseptic culture of plant cells, tissues,
organs and plants. This course seeks to familiarize students
to the basic principles of tissue culture, and to expose them
to its many applications. The theory, production and societal
implications of transgenic plants will be discussed. The laboratory
will be used to illustrate principles discussed in lectures.
Students will give oral presentations (10 min) in groups of
2 on applied aspects of plant tissue culture. The lab will
provide hands on experience in many of the methods discussed.
Course Objectives: Part I.
- To introduce the underlying principles of
aseptic culture of plant cells, tissues and organs.
- To provide information about the equipment,
procedures and terminology of aseptic culture.
- To review specialized cell culture techniques
and their uses in plant science research and industry.
- To familiarize students with the technology
of plant genetic engineering.
- To introduce tissue culture techniques
and demonstrate the principles of tissue culture through
student participation in the laboratory.
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