Table 1. Annotations and BLAST scores for cacao leaf unigene sequences representing candidate genes whose expression is upregulated by inducers of the defense response. Sequences are grouped by functional categories. Accession numbers beginning with “At” correspond to sequences from Arabidopsis and can be accessed at TAIR (http://www.arabidopsis.org). TIGR accession numbers are preceeded by a three letter code that indicates the plant gene index from which the sequence was retrieved. (ath) Arabidopsis, (gar) cotton, (gma) soybean, (han) sunflower, (les) tomato, (lja) lotus, (lsa) lettuce, (mcr) ice plant, (mtr) Medicago, (osa) rice, (stu) potato, (tae) wheat, (zma) maize. The TIGR Plant Gene Indecies can be accessed at the following web site (http://www.tigr.org/tdb/tgi/plant.shtml).

Name

EST Accession #

E value

Accession/BLAST Hit

BLAST Annoation

Functional Category

>Contig 1

no significant hits

PSU_np11 G05

CF974378

PSU_nupup2.2 G09

CF974691

>Contig 2

7.00E-36

At1g67280

glyoxalase I

Metabolism

PSU_np12 H07

CF974440

PSU_npl-a2 E12

CF974545

>Contig 3

2.00E-11

At3g27200

blue copper protein

Cell Rescue, Defense, Cell Death and Ageing

PSU_blon3-2 D07

CF973384

PSU_blon3-2 B07

CF973375

>Contig 4

2.00E-87

gar_TC9056

manganese superoxide dismutase 1

Cell Rescue, Defense, Cell Death and Ageing

PSU_weup3.2C06

CF974727

PSU_weup3.2D05

CF974737

>Contig 5

9.00E-85

ath_TC167972

DRH1 DEAD box protein-like

Transcription

PSU_weup3.2C12

CF974733

PSU_weup3.2B04

CF974713

PSU_meup2.1 B08

CF974137

PSU_weup3.2A11

CF974709

PSU_me9.3 H10

CF974035

>Contig 6

2.00E-50

ath_TC155834

At2g40320

unknown

PSU_blon3-2 B05

CF973373

PSU_blon3-2 F05

CF973393

>Contig 7

6.00E-05

ath_TC167595

CPR5

Cell Rescue, Defense, Cell Death and Ageing

PSU_bifour G11

CF973274

PSU_bifour A05

CF973208

>Contig 8

9.00E-39

gar_AI055215

unknown protein

unknown

PSU_nep1 G02

CF974265

PSU_nep1 D04

CF974231

>Contig 9

4.00E-54

stu_TC49650

unknown protein

unknown

PSU_me9.3 D02

CF973983

PSU_me9.3 B06

CF973966

>Contig 10

3.00E-24

ath_TC158123

Preprotein translocase secA subunit

Protein Destination

PSU_me9.3 D03

CF973984

PSU_me8.3 G09

CF973935

>Contig 11

8.00E-20

ath_TC149445

photosystem II protein X precursor

Energy

PSU_meup2.1 F11

CF974174

PSU_me8.3 A03

CF973863

PSU_2b1c F09

CF972790

>Contig 12

5.00E-29

gma_TC137684

pasticcino 1 protein

Cell Growth, Cell Division and DNA Synthesis

PSU_2blonup1C01

CF972925

PSU_2b1-a C07

CF972667

>Contig 13

0

mtr_TC52887

DNA-directed RNA polymerase

Transcription

PSU_meup2.1 E12

CF974163

PSU_meup2.1 A02

CF974121

PSU_weup3.2C04

CF974725

>Contig 14

2.00E-19

mtr_TC51636

aminopeptidase

Protein Destination

PSU_2db1b C07

CF972989

PSU_2db1b B10

CF972981

>Contig 15

7.00E-79

gma_TC122012

amine oxidase

Metabolism

PSU_blon3-2 B03

CF973371

PSU_bifour F07

CF973260

>Contig 16

4.00E-67

stu_TC53574

auxin growth promotor protein

Transcription

PSU_me6.3 B08

CF973816

PSU_me6.3 E09

CF973836

>Contig 17

2.00E-49

ath_TC161028

glucose transporter

Transport Facilitation

PSU_bifour D12

CF973244

PSU_bltwo C06

CF973541

>Contig 18

2.00E-60

lja_TC1985

hypothetical protein F3A4.20

unknown

PSU_npl-a2 C11

CF974541

PSU_np12 D07

CF974412

>Contig 19

1.00E-60

les_TC98302

O-linked GlcNAc transferase

Metabolism

PSU_meup2.1 A01

CF974120

PSU_meup2.1 B07

CF974136

>Contig 20

8.00E-37

At5g01720

F-box protein

Protein Destination

PSU_bithree A08

CF973294

PSU_bithree B10

CF973302

>Contig 21

2.00E-94

At2g14170

methylmalonate semi-aldehyde dehydrogenase

Metabolism

PSU_nupup1.2 E09

CF974624

PSU_nep1 C11

CF974226

>Contig 22

3.00E-81

gma_TC122455

BEL1-like homeobox 4

Transcription

PSU_boln5 F10

CF973616

PSU_bithree E12

CF973338

>Contig 23

1.00E-54

lsa_TC2341

3-oxoacyl-[acyl-carrier protein] reductase

Metabolism

PSU_me5.3 C11

CF973781

PSU_me5.3 D11

CF973786

PSU_me5.3 B12

CF973774

>Contig 24

4.00E-15

At1g76510

ARID DNA binding domain

Transcription

PSU_meup1 H04

CF974112

PSU_meup1 G06

CF974102

>Contig 25

4.00E-87

gma_TC132746

caffeoyl-CoA O-methyltransferase

Cellular Organization

PSU_2blonup1D05

CF972935

PSU_nep1 H07

CF974281

>Contig 26

5.00E-66

gar_TC9184

GTP-binding protein

Cellular Communication/Signal Transduction

PSU_2blon_E06_blon4-7_040

CF972901

PSU_2blon_E07_blon4-7_052

CF972902

>Contig 27

2.00E-69

gar_TC12144

Lil3 protein

Metabolism

PSU_blonup4-7_C01_blon4-7_003

CF973485

PSU_nep1 D07

CF974234

>Contig 28

1.00E-44

At3g58560

Carbon Catabolite Repressor Protein 4

Transcription

PSU_2b1-a G04

CF972711

PSU_2b1d (2)H11

CF972813

>Contig 29

no significant hits

PSU_bifour G07

CF973271

PSU_blone F05

CF973448

>Contig 30

2.00E-79

gma_TC132909

12-oxophytodienoate reductase OPR2

Metabolism

PSU_meup2.1 G04

CF974179

PSU_meup2.1 G08

CF974183

PSU_meup1 C12

CF974065

PSU_meup2.1 H09

CF974195

>Contig 31

5.00E-29

At2g02955

myb family protein

Transcription

PSU_bc2.2 E11

CF973093

PSU_bc3 C01

CF973145

>Contig 32

4.00E-10

hvu_TC27353

peptidylprolyl isomerase

Protein Destination

PSU_2db1b

CF972959

PSU_2db1b G04

CF973031

>Contig 33

e-102

mtr_TC43602

sugar transporter protein

Cellular Transport and Transport Mechansims