Centre
for Cocoa Studies, M&M MARS, BRASIL: In collaboration with the MARS company
we have established a large number of somatic embryo plants in the field in
Bahia, Brazil. Additionally, Dr. Smilija Lambert of Mars Inc. in
collaboration with researchers from Penn State (Lambert et al., 2000) completed
work performed at the Almirante Centre for Cocoa Studies. She has developed a
system for downstream propagation of somatic embryo plants referred to as
mini-cuttings. For the experiments, Dr. Lambert team used small tissue cultured
plantlets, acclimated to greenhouse conditions, as sources of apical cuttings
which were then rooted and grown in the greenhouse for three to four months
after which they were ready for transfer to the field. Very high success rates
were obtained. This is another system that provides a rapid way to produce
large numbers of orthotropic plantlets at a lower cost than tissue culture
alone.