United States Patent
6,255,466, Cosgrove , et
al. July 3, 2001
Purified plant expansion
proteins and DNA encoding same
Abstract: The
sequence is disclosed both as the nucleic acid sequence SEQ ID NO: 1 and as the
corresponding amino acid sequence SEQ ID NO: 7. Five other amino acid sequences
are also disclosed, two from rice and three from Arabidopsis, SEQ ID NO: 2
through SEQ ID NO:6, respectively.
Inventors: Cosgrove; Daniel J. (State College, PA); McQueen-Mason;
Simon (York, GB); Guiltinan; Mark (State College, PA); Shcherban; Tatyana
(State College, PA); Shi; Jun (State College, PA)
Assignee: The Penn State Research Foundation (University Park, PA)
Appl. No.: 092160
Filed: June 5, 1998
United States Patent
6,197,587, Guiltinan ,
et al. March 6, 2001
Abstract: The present
invention relates to improved methods of (i) inducing somatic embryogenesis
from cacao tissue explants and (ii) regenerating cacao plants from somatic
embryos. The invention further relates to cacao somatic embryos and plants
obtained according to the methods of the invention. Novel tissue culture media
adapted for use in the above-identified methods are also within the scope of
the invention. The novel media of the invention include primary callus growth
medium, secondary callus growth medium, embryo development medium, primary
embryo conversion medium, secondary embryo conversion medium and plant
regeneration medium.
Inventors: Guiltinan; Mark J. (State College, PA); Li; Zhijian
(Tabares, FL); Traore; Abdoulaye (State College, PA); Maximova; Siela (State
College, PA)
Assignee: The Penn State Research Foundation (University Park, PA)
Appl. No.: 105648
Filed: June 26, 1998
United States Patent 6,150,587, Guiltinan
, et al. November 21, 2000
Abstract: The present
invention relates to improved methods of transforming cacao tissues with
Agrobacterium vectors and regenerating transgenic plants. The invention further
relates to transgenic cacao somatic embryos and plants obtained according to
the methods of the invention. Novel tissue culture media adapted for use in the
above-identified methods are also within the scope of the invention. The novel
media of the invention include primary callus growth medium, secondary callus
growth medium, embryo development medium, primary embryo conversion medium,
secondary embryo conversion medium and plant regeneration medium.
Inventors: Guiltinan; Mark J. (State College, PA); Li; Zhijian
(Tabares, FL); Traore; Abdoulaye (State College, PA); Maximova; Siela (State
College, PA)
Assignee: The Penn State Research Foundation (University Park, PA)
Appl. No.: 105617
Filed: June 26, 1998
United States Patent 5,959,082, Cosgrove,
et al. September 28, 1999
Proteins catalyzing the extension of plant cell walls
Abstract: A new class
of proteins and methods related thereto are presented. The proteins, which can
be characterized as catalysts of the extension of plant cell walls and the
weakening of the hydrogen bonds in pure cellulose, are referred to as expansins.
Two proteins have been isolated by fractionation techniques from washed wall
fragments of cucumber hypocotyls, referred to as "cucumber
expansin-29" and "cucumber expansin-30" (abbreviated cEx-29 and
cEx-30, with respect to their apparent relative masses as determined by
SDS-PAGE). Moreover, three peptide fragments from the purified cEx-29 protein
were sequenced, then oligonucleotide primers were designed to amplify a portion
of the expansin cDNA using polymerase chain reaction with a cDNA template derived
from cucumber seedlings, and then the PCR fragment was used to screen a cDNA
library to identify full length clones. Another expansin protein has been
isolated from oat coleoptiles (oat expansin oEx-29), while three additional
expansin sequences have been identified in Arabidopsis and an additional two in
rice. Expansins appear to be broadly distributed throughout the plant kingdom
and can be identified in stem and leaf vegetables (i.e., broccoli, cabbage),
fruit and seed vegetables (i.e., tomato), fiber crops and cereals (i.e., corn),
and forest and ornamental crops (i.e., cotton). An expansin, generally, is a
protein which has at least about 60% sequence similarity with the amino acid
sequence shown in SEQ. ID. NO:1, and preferably has at least about 70% sequence
similarity with SEQ. ID. NO:1.
Inventors: Cosgrove; Daniel J. (State College, PA); McQueen-Mason;
Simon (York, GB); Guiltinan; Mark (State College, PA); Shcherban; Tatyana
(State College, PA); Shi; Jun (State College, PA)
Assignee: The Penn State Research Foundation (University Park, PA)
Appl. No.: 440517
Filed: May 12, 1995