Lisa Renshaw-Gegg

 

Amgen Inc.                                                                                                                                               487 Delwood Court

One Amgen Center Drive, 14-2-C                                                                                                 Newbury Park, CA 91320

Thousand Oaks, CA 91320                                                                                                                (805) 499-6021

(805) 447-1737

                                                                                 E-mail: lisarg@amgen.com

 

Experience:

 

•Jan. 1996-             Associate Scientist I, Gene Expression Technology,

Present                    Amgen Inc., Thousand Oaks, CA

 

Provide tissue culture / technical support in the development of novel and/or improved mammalian protein expression systems. Analyze conditioned media for expression levels. Identify and establish new, improved protocols. Generate cell lines to be scaled up for protein production in both research and manufacturing contexts. Assist in cloning of protein expression constructs. Provide data, figures and text for in-house presentations and product documentation. Maintain frozen inventory of stock cell lines and expressing cell lines. Mammalian cell culture, transient and stable transfection (Electroporation, Lipofection, Calcium Phosphate), Western Blots, large scale Roller and Spinner bottle cultures, Applikon Bioreactor Cultures, Methotrexate amplification of protein expression, FACS analysis, DNA cloning, plasmid preps, PCR. Computer experience: Microsoft Word, Excel, Powerpoint, Clarisdraw, and Cricket Graph.

 

•Nov.1993-  Research Support Assistant/Lab Manager, Department of Horticulture/

 Jan.1996       Biotechnology Institute, The Pennsylvania State University, University Park, PA

 

Conduct research to define the functional domain of DNA binding proteins.  Generate data, figures and text to be used in manuscript preparation.  DNA cloning, protein electrophoresis, electrophoretic mobility shift assays (EMSA), Southwestern blotting, labeling DNA probes, 35S DNA sequencing, affinity chromatography, bacterial electroporation, PCR, PCR-directed mutagenesis (overlap extension and megaprimer), transcription-translation assays using PCR product and plasmid templates, phosphorimagery. Other responsibilities: train and supervise undergraduate researchers, oversee general operation of laboratory facilities and equipment, provide expertise in laboratory techniques, conduct lab meetings, monitor research funds, maintain computer records of lab protocols, organize lab inventory systems, maintain radioisotope inventory and monitor usage.  Macintosh computer experience: Microsoft Word, Microsoft Excel, OLIGO, DNAstar, Amplify, ImageQuant, MacDraw Pro, Adobe Photoshop, Cricket Graph, and Canvas.

 

•Aug.1989-  Staff Research Associate II, Department of Medical Pathology

 Oct. 1993     University of California, Davis, CA

 

Provided technical support for research involving gene regulation in feline and simian retroviruses (FIV, FeLV, SFV).  Maintenance of mammalian cell cultures (ongoing and frozen stocks), establishing primary cell cultures from whole blood, mycoplasma testing, transfection (electroporation and DEAE-mediated) of plasmid DNA into mammalian cell lines for transient expression assays (Chloramphenicol Acetyl Transferase), T-cell Activation, Reverse Transcriptase assays, IFA, ELISA, virus propagation, DNA cloning, plasmid preps, Southern blots, and operation of a Pharmacia Gene Assembler (deoxyoligonucleotide synthesizer). Managerial responsibilities involved summarizing experimental results using Excel and Microsoft Word, maintaining supply inventories, training of personnel, monitoring of radioisotope usage (swipe tests) and disposal (inventory).

 

•July1986-                Scientist, Veterinary Research and Development

 Aug.1989     Synbiotics Corporation, San Diego, CA

 

Responsible for the research and development of veterinary diagnostic immunoassays using a variety of solid phase matrices and sample types (ELISA, Immunofiltration, dipstick).  Duties included FPLC purification of antibodies, western blotting to determine antibody specificity, preparation of enzyme-antibody conjugates, assay optimization using statistical analysis, production and testing of pilot lots, generating data for USDA submission, and product transfer to process development via a formal report of methods and formulations utilized.

 

•Feb.1981- Research Technician II, Basic and Clinical Research-Hematology Division

 July1986     Scripps Clinic and Research Foundation, La Jolla, CA

 

Production of monoclonal antibody producing cell lines.  Responsible for all phases of mouse, rat and human hybridoma production: immunization, fusion, screening, ascites production, and antibody purification.

Processing and treatment of human bone marrow for autologous and heterologous transplantation: isolation of mononuclear cells, antibody + complement mediated lysis of T-cells, and cryopreservation for later reinfusion.  Evaluation of cell subset populations by IFA and culture of granulocyte/macrophage colonies on soft agar.

Hematological patient studies on anti-platelet autoimmune disorders (Immunothrombocytic Purpura).

Other skills and responsibilities: PAGE, Western blotting, routine cell culture and cryopreservation of established mammalian cell lines, EBV transformation of cells, isolation of specific human cell types from whole blood or spleen, RIA, ELISA, gel filtration, and affinity chromatography.

 

 

 

 

Publications:

 

Niu,X.,Renshaw-Gegg, L., Miller, L., and Guiltinan, M.J. (1998)

Bipartite determinants of DNA binding specificity of plant basic leucine zipper proteins.

Plant Molecular Biology: in Press

 

Simonet-WS. Lacey-DL. Dunstan-CR. Kelley-M. Chang-MS. Luethy-R. Nguyen-HQ.

Wooden-S. Bennett-L. Boone-T. Shimamoto-G. DeRose-M. Elliott-R. Colombero-A.

Tan-HL. Trail-G. Sullivan-J. Davy-E. Bucay-N. Renshaw-Gegg-L. Hughes-TM.

Hill-D. Pattison-W. Campbell-P. Boyle-WJ. et al.(1997)

Osteoprotegerin: a novel secreted protein involved in the regulation of bone density.

CELL. 89.2.:309-319.      

 

Renshaw-Gegg, L., and Guiltinan, M.J. (1996)

                              Optimization of PCR template conditions for in vitro  transcription-translation reactions.

                              Biotechnology Letters, 18:679-682

 

                              Campbell, M., Renshaw-Gegg, L., Renne, R., and Luciw, P. (1994)

                              Characterization of the Internal Promoter of Simian Foamy Viruses.

                              Journal Of Virology, 68, 4811-4820

 

                              Mergia, A., Renshaw-Gegg, L.W., Stout, M.W., Renne, R., and Herchenroeder, O. (1993)

                              Functional Domains of the Simian Foamy Virus Type 1 Transcriptional Transactivator (taf).

                              Journal Of Virology, 67, 4598-4604

 

                              Renne, R., Mergia, A., Renshaw-Gegg, L.W., Neumann-Haefelin, D., and Luciw, P. (1993)

                              Regulatory Elements in the Long Terminal Repeat (LTR) of Simian Foamy

                              Virus Type 3 (SFV-3).

                              Virology, 192, 365-369

 

                              Mergia, A., Pratt-Lowe, E., Shaw, K.E.S., Renshaw-Gegg, L.W., and Luciw, P. (1992)

                              cis-Acting Regulatory Regions in the Long Terminal repeat of Simian Foamy Virus Type 1.

                              Journal Of Virology, 66, 251-257

 

                              Sparger, E.E., Shacklett, B.L., Renshaw-Gegg, L.W., Barry, P., Pedersen, N.C., Elder, J.,

                              and Luciw, P. (1992)

                              Regulation of Gene Expression Directed by the Long Terminal Repeat of Feline

                              Immunodeficiency Virus.

                              Virology, 187, 165-177

 

                              Thompson, L.F., Renshaw, L., and McMillan, R.

                              The Use of Monoclonal Antibodies for T-cell Depletion to Prevent Graft Versus Host Disease.

                              Clinical Use of Monoclonal Antibodies in cancer Therapy  and Imaging: Current Status,                                                                         Controversies and Future Directions.

 

                              McMillan, R., Tani, P., Millard, F., Berchtold, P., Renshaw, L., and Woods, V.L. (1987)

                              Platelet-associated and Plasma Autoantibodies in Chronic ITP.

                              Blood, 70, 1495-1499

 

 

Lisa Renshaw-Gegg

 

Education:

 

•June1980       B.S. Animal Science; University of California, Davis, CA

 

 

 

References:

                              Mark J. Guiltinan, Ph.D., Professor of Plant Molecular Biology,

                              Department of Horticulture/ Biotechnology Institute, The Pennsylvania State University,

                              University Park, PA  16802. (814) 863-7957

 

                              Paul A. Luciw, Ph.D., Professor, Department of Medical Pathology/ Center for Comparative Medicine,

                              University of California, Davis, CA  95616. (916) 752-3430

 

                              Ellen E. Sparger, D.V.M., Ph.D., Assistant Adjunct Professor, Department of Medicine, School

                              of Veterinary Medicine, University of California, Davis, CA 95616. (916) 752-8477

 

                              Karen E.S. Shaw, Staff Research Associate III/Supervisor, Department of Medical Pathology,

                              University of California, Davis, CA  95616. (916) 752-8459

 

                              Avril F. Bland, Scientist/Supervisor, Veterinary Research and Development, Synbiotics Corp.,

                              11011 Via Frontera, San Diego, CA  92127. (619) 451-3771, ext. 151

 

               `              Robert McMillan, M.D., Director of Hematology, Scripps Clinic and Research Foundation,

                              10666 North Torrey Pines Road, La Jolla, CA  92037. (619) 455-9100, ext. 8632